A MLSFH Cohort Profile, published in the International Journal of Epidemiology, summarizes the key findings of the MLSFH. The MLSFH Cohort Profile also provides information about the sampling for the MLSFH, the refreshment of the MLSFH sample over time, and the procedures for HIV testing and counseling that were implemented as part of the MLSFH. It also reports comparisons of the MLSFH study populations with nationally representative datasets, analyses of attrition in the MLSFH sample, and it includes discussions of some specific features of the MLSFH data that have been widely used across many MLSFH-based papers.