Pohlschroder Lab

Prokaryotic Cell Surface Biogenesis

Publications

PUBLICATIONS (or check Google Scholars link)

  1. Egan M., Hogan K., Maupin-Furlow J., Pohlschroder M. 2022. The Best of Both World: Discovery-Driven Learning through a lab-seminar approach. J Microbiol Biol Educ. 23(3):e00031-22.
  2. Schiller H., Young C., Schulze S., Pohlschroder M. 2022. Accessible and Insightful Scientific Learning Experiences Using the Microorganism Haloferax volcanii. Methods Mol Biol. 2522:531-545.
  3. Schulze S.*, Schiller H.*, Mutan Z. *, Solomonic J., Telhan O., Pohlschroder M. 2022. Cost-Effective and Versatile Analysis of Archaeal Surface Adhesion Under Shaking and Standing Conditions. Methods Mol Biol. 2522:397-406.
  4. Mutan Z.*, Schiller H.*, Schulze S.*, Pohlschroder M. 2022. Immersed Liquid Biofilm and Honeycomb Pattern Formations in Haloferax volcanii. Methods Mol Biol. 2522:387-395.
  5. Schulze S., Pohlschroder M. 2022. Proteomic Sample Preparation and Data Analysis in Line with the Archaeal Proteome Project. Methods Mol Biol. 2522:287-300.
  6. Schiller H., Young C., Schulze S., Tripepi M., Pohlschroder M. 2022. A Twist to the Kirby-Bauer Disk Diffusion Susceptibility Test: an Accessible Laboratory Experiment Comparing Haloferax volcanii and Escherichia coli Antibiotic Susceptibility to Highlight the Unique Cell Biology of Archaea. J Microbiol Biol Educ. 23(1):e00234-21.
  7. Schulze S.*, Schiller H.*, Solomonic J., Telhan O., Costa K., Pohlschroder M. 2022. Advanced Understanding of Prokaryotic Biofilm Formation through Use of a Cost-Effective and Versatile Multipanel Adhesion (mPAD) Mount. Appl Environ Microbiol. 88(4):e0228321.
  8. Schulze S., Pfeiffer F., Garcia B.A., Pohlschroder M. 2021. Comprehensive glycoproteomics shines new light on the complexity and extent of glycosylation in archaea. PLoS Biol. 19(6):e3001277.
  9. Schulze S., Igiraneza A.B., Kosters M., Leufken J., Leidel S.A., Garcia B.A., Fufezan C., Pohlschroder M. 2021. Enhancing Open Modification Searches via a Combined Approach Facilitated by Ursgal. J Proteome Res. 20(4):1986-1996.
  10. de Silva R.T., Abdul-Halim M.F., Pittrich D.A., Brown H.J., Pohlschroder M., Duggin I.G. 2021. Improved growth and morphological plasticity of Haloferax volcanii. Microbiology. 167(2):001012.
  11. Collins M., Afolayan S., Igiraneza A.B., Schiller H., Krespan E., Beiting D.P., Dyall-Smith M., Pfeiffer F., Pohlschroder M. 2020. Mutations Affecting HVO_1357 or HVO_2238 Cause Hypermotility in Haloferax volcanii, Suggesting Roles in Motility Regulation. Genes. 12(1):58.
  12. Schiller H.*, Schulze S.*, Mutan Z., de Vaulx C., Runcie C., Schwartz J., Rados T., Filho A.W.B., Pohlschroder M. 2020. Haloferax volcanii Immersed Liquid Biofilms Develop Independently of Known Biofilm Machineries and Exhibit Rapid Honeycomb Pattern Formation. mSphere. 5(6):e00976-20.
  13. Schulze S., Oltmanns A., Fufezan C., Kragenbring J., Mormann M., Pohlschroder M., Hippler M. 2020. SugarPy facilitates the universal, discovery-driven analysis of intact glycopeptides. Bioinformatics. btaa1042.
  14. Schulze S., Adams Z., Cerletti M., De Castro R., Ferrierra-Cerca S., Fufezan C., Gimenez M.I., Hippler H., Jevtic Z., Knuppel R., Legerme G., Lenz C., Marchfelder A., Maupin-Furlow J., Paggi R.A., Pfeiffer F., Poetsch A., Urlaub H., Pohlschroder M. 2020. The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics. Nat Commun. 11(1):3145.
  15. Abdul-Halim M.F., Schulze S., DiLucido A., Pfeiffer F., Filho A.W.B., Pohlschroder M. 2020. Lipid Anchoring of Archaeosortase Substrates and Midcell Growth in Haloarchaea. mBio. e00349-20.
  16. Legerme G., Pohlschroder M. 2019. Limited Cross-Complementation Between Haloferax volcanii PilB1-C1 and PilB3-C3 Paralogs. Front Microbiol. 10:700.
  17. Pohlschroder M., and Schulze S. 2018. Haloferax volcanii. Microbe of the Month. Trends in Microiology. 27, 86-87.
  18. Albers S and Pohlschroder M. 2018. Editorial: Archaea. FEMS Microbial. Rev. 42. 694-717
  19. Abdul Halim MF, R, Rodriguez JD Stoltzfus, IG Duggin, M Pohlschroder. 2018. Conserved residues are critical for Haloferax volcaniiarchaeosortase catalytic activity: Implications for convergent evolution of the catalytic mechanisms of non-homologous sortases from archaea and bacteria. Mol. Micro. 108:276-287.
  20. Pohlschroder M., S. Schulze, F. Pfeiffer and MF Abdul Halim. 2018. Archaeal Cell Surface Biogenesis. 2018. FEMS Microbial. Rev. 694-717.
  21. Wu L., M. Gingery, D. Arambula, E. Czornyj, S. Handa, H. Khan, M. Liu, M. Pohlschroder, KL Shaw, A. Du, H. Guo, P. Ghosh, JF Miller, S. Zimmerly. 2018. Diversity-generating retroelements: natural variation, classification and evolution inferred from a large-scale genomic survey. Nucl. Acids Res. 9:11-24.
  22. Halim, MFA, J. Stolzfus, S. Schulze, M. Hippler and M. Pohlschroder. 2017. ArtA-dependent processing of a Tat substrate containing a conserved tripartite structure that is not localized at the C-terminus. J. Bacteriol. 199 e00802-16
  23. Kouassi J.E., Waldron I, Tripepi, M and M. Pohlschroder. 2017. Laboratory Activity to Promote Student Understanding of UV Mutagenesis and DNA Repair. JMBE. doi: 10.1128/jmbe.v18i1.1202
  24. Legerme G., E. Yang, R. Esquivel, S. Kiljunan, H. Savilathi and M. Pohlschroder. 2016. Screening of a Haloferax volcanii transposon library reveals novel motility and adhesion mutants. Life.6:1-14.
  25. Esquivel, R., S. Schulze, M. Hippler and M. Pohlschroder. 2016. Identification of Haloferax volcanii flagellin and pilin N-glycans with diverse roles. J. Biol. Chem. 291:10602-14.
  26. Pohlschroder, M. and S. Albers. 2016. Editorial: Archaeal cell envelope and surface structures. Front. Microbiol. 6:1515.
  27. Abdul Halim, F., K. Karch, Y. Zhou, B. Garcia and M. Pohlschroder. 2016. Permuting the PGF-CTERM signature motif blocks both archaeosortase-dependent C-terminal cleavage and prenyl lipid attachment for the Haloferax volcanii S-layer glycoprotein. J. Bacteriol. 198:808-815.
  28. Pohlschroder, M. and R. Esquivel. 2015. Archaeal type IV pili and their roles in biofilm formation. Front. in Microbiol. (invited review).6:190.
  29. Kiljunen, S., M. Pujunen, K. Dilks, S. Storf, M. Pohlschroder and H. Savilahti. 2014. Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii, and its use for gene discovery. BMC Biol. 103.
  30. Esquivel R. and M. Pohlschroder. 2014. A conserved type IV pilin signal peptide H-domain is critical for the post-translational regulation of flagella-dependent motility. Mol. Microbiol. 93:493-504.
  31. Esquivel, R, R. Xu and M. Pohlschroder. 2013. Novel, archaeal adhesion pilins with a conserved N-terminus. J. Bacteriol. 195:3808-3818.
  32. Abdul Halim, M. F, F. Pfeiffer, J. Zou, A. Frisch, D. Haft, S. Wu, N. Tolic, H. Brewer, S. H. Payne, L. Paša-Tolic, and M. Pohlschroder. 2013. Haloferax volcanii archaeosortase is required for motility, mating and C-terminal processing of the S-layer glycoprotein. Mol. Microbiol. 85:1164-1175.
  33. Tripepi, M., R. Esquivel and M. Pohlschroder. 2013. Haloferax volcanii cells lacking the flagellin, FlgA2, are hypermotile. Microbiol. 159:2249-2258.
  34. Tripepi, M., J. You, S. Temel, O. Onder, D. Brisson, andM. Pohlschroder. 2012. N-glycosylation of Haloferax volcanii flagellins is essential for flagella-biosynthesis. J. Bacteriol. 194:4876-4887.
  35. Szabo, Z. andM. Pohlschroder. 2012.  Diversity and subcellular distribution of archaeal secreted proteins. Invited review. Front. in Microbiol. doi: 10.3389/fmicb. 00207.
  36. Cuadros-Orellana , M. Pohlschroder, M. Grossman, and LR. Durrant. 2012. Biodegradation of aromatic compounds by a halophilic archaeon isolated from the Dead Sea. Chemical Engineering Transactions. 27:1974-9791.
  37. Imam S., Z. Chen, D.S. Roos, and M. Pohlschroder. 2011. Identification of Surprisingly Diverse Type IV Pili, across a Broad Range of Gram-Positive Bacteria. PloS One 6(12):e28919.
  38. Tripepi, M., M. Pohlschroder, and M.B. Bitonti. 2011. Diversity of Dehydrins in Oleae europaea Plants Exposed to Stress. The Open Plant Sci. J. 5:9-13.
  39. Pohlschroder, M., A. Ghosh, M. Tripepi, and S. Albers. 2011. Archaeal Type IV Pilus-Like Structures – Ancient Prokaryotic Surface Organelles. Invited Review. Curr. Opin. in Microbiol. 4(3):357-63.
  40. Storf S., F. Pfeiffer2#, K. Dilks1, Z. Chen, Imam, and M. Pohlschroder. 2010. Mutational and bioinformatic analysis of haloarchaeal lipoproteins. Archaea. 2010 Article ID 75410.
  41. Tripepi, M., S. Imam, and M. Pohlschroder. 2010. Haloferax volcanii flagella are required for motility but are not involved in PibD-dependent surface adhesion. J. Bacteriol. 192. 3093-02.
  42. Hartman AL, Norais C, Badger JH, Delmas S, Haldenby S, Madupu R, Robinson J, Khouri H, Ren Q, Lowe TM, Maupin-Furlow J, Pohlschroder M, Daniels C, Pfeiffer F, Allers T, Eisen JA. The complete genome sequence of Haloferax volcanii DS2 a model archaeon. PloS One 19;5(3):e9605.
  43. Albers S. and M. Pohlschroder. 2009. Diversity of archaeal type IV pilin-like structures. Invited Review. Extremophiles. 13. 403-410.
  44. Gimenez M. I., K. Dilks, and M. Pohlschroder. 2007. Haloferax volcanii twin arginine translocation substrates include soluble secreted, C-terminally anchored and lipoproteins. Mol. Microbiol. 66:1597-1602.
  45. Szabo, Z., A. Oliveira Stahl, S. Albers, J. Kissinger, A. Driessen, and M. Pohlschroder. Identification of diverse archaeal proteins with class III signal peptides cleaved by distinct archaeal prepilin peptidases, J. Bacteriol. 189:772-778.
  46. Widdick, D., K. Dilks, G. Chandra, A. Bottrill, M. Naldrett, M. Pohlschroder, and T. Palmer. 2006. Secretome analysis reveals that the twin-agrinine translocation (Tat) pathway is a major route of protein export in Streptomyces coelicolor. Proc. Natl. Acad. Sci. USA, 106:17927-1793
  47. Cuadros-Orellana, S, M. Pohlschroder, and L. R. Durrant. 2006. Isolation and characterization of halophilic archaea able to grow in aromatic compounds. Int. Biodeter. & Biod. 57:151-154.
  48. Hand, N. J., A. Laskewitz, R. Klein, and M. Pohlschroder. 2005. Archaeal and bacterial SecD and SecF homologs exhibit striking structural and functional conservation. J. Bacteriol. 188. 1251-1259.
  49. Dilks, K., M. I. Gimenez, and M. Pohlschroder. 2005. Genetic and biochemical analysis of the twin-arginine translocation pathway in halophilic archaea. J. Bacteriol. 187: 8104-8113.
  50. Pohlschroder, M., I. Gimenez and K. Jarrell. 2005. Protein transport in Archaea: Sec and Twin arginine translocation pathways. Invited review, Curr. Opin. in Microbiol. 8.713-719.
  51. Haddad, A., R. W. Rose, and M. Pohlschroder. 2005. The Haloferax volcanii FtsY homolog is critical for haloarcheal growth but does not require the A-domain. J. Bacteriol. 187:4015-4022. (ASM-journal highlight).
  52. Pohlschroder, M., E. Hartmann, N. J. Hand, K. Dilks, and A. Haddad. 2005. Diversity and evolution of protein translocation. Invited review, Ann. Rev. of Microbiol. 59:91-111.
  53. Pohlschroder, M., K. Dilks, N. Hand, and R. W. Rose. 2004. Protein translocation across the archaeal cytoplasmic membrane. Invited review, FEMS Microbiol. Rev. 28:3-24.
  54. Dilks, K., W. R. Rose, E. Hartmann, and M. Pohlschorder. 2003. Prokaryotic use of the twin arginine translocation pathway: A Genomic Survey. J. Bacteriol. 185:1478-1483.
  55. Rose, R.W., T. Brüser, J.C. Kissinger, and M. Pohlschroder. 2002. Adaptation of protein secretion to extremely high salt concentrations by extensive use of the twin arginine translocation pathway. Mol. Microbiol. 5: 943-950.
  56. Rose, R.W. and M. Pohlschroder. 2002. In vivo analyses of an essential archaeal signal   recognition particle in its native host. J. Bacteriol. 184:3260-3267.
  57. Ng WV, et al. 2000. Genome sequence of a genetically tractable and extremely halophilic archaeon, Halobacterium sp. NRC-1. Proc. Natl. Acad. Sci. USA 97:12176-12181.
  58. Rusterholtz, K. and M. Pohlschroder. 1999. Where are the limits of life?  Cell. 96: 469-470. (not peer-reviewed).
  59. Pohlschroder, M., W. Prinz, E. Hartmann, and J. Beckwith.  1997. Protein translocation in the three domains of life: variations on a theme.  Cell.  91:563-566.
  60. Pohlschroder, M., C. Murphy, and J. Beckwith.   In vivo analyses of interactions between SecE and SecY, core components of the Escherichia coli protein translocation machinery. J. Biol. Chem.  271:19908-19914.
  61. Murphy, C., W. Prinz, M. Pohlschroder, A. Derman, and J. Beckwith. 1996.  Essential features of the pathway for protein translocation across the Escherichia coli cytoplasmic membrane.  Cold Spring Harbor Symp., Quant. Biol. 60:277-283.
  62. Pohlschroder, M., S. B. Leschine, and E. Canale-Parola.   Ultrastructural analyses of the multicomplex cellulase-xylanase system of Clostridium papyrosolvens.   J. Bacteriol. 177:6625-6629.
  63. Pohlschroder, M., S. B. Leschine, and E. Canale-Parola.   Regulation of the multicomplex cellulase-xylanase system of Clostridium papyrosolvens, p.86-94.  In K. Shimada, K. Ohmiya, Y. Kobayashi, S. Hoshino, K. Sakka, S.Karita (ed.), Genetics, biochemistry and ecology of lignocellulose degradation, Uni Publishers, Tokyo.
  64. Pohlschroder, M., S. B. Leschine, and E. Canale-Parola.   The multi-complex cellulase system of Clostridium papyrosolvens strain C7.  J. Bacteriol. 176:80-86.
  65. Pohlschroder, M., S. B. Leschine, and E. Canale-Parola.   Spirochaeta caldaria, sp.nov., a thermophile from freshwater hot springs that enhances cellulose degradation by Clostridium thermocellum.  Arch. Microbiol.  161:17-24.

BOOKS/BOOK CHAPTERS

  1. Pohlschroder, M. and SA Albers. 2015. Archaeal cell envelope and surface structures. e-book. Frontiers of Microbiology.
  2. Dilks K., M.I. Gimenz, M. Tripepi, and M. Pohlschroder. 2011. Protein transport into and across haloarchaeal cytoplasmic membranes. Halophiles and Hypersaline Environments: Current Research and Future Trends. Editors: A. Ventosa, A. Oren, Y Ma, Springer Verlag.
  3. Pohlschroder, M. and K. Dilks. 2007. Archaeal membrane protein insertion and protein secretion. Archaea: Molecular and Cellular Biology. Editor: Ricardo Cavicchioli, ASM-Press.

PATENTS

  1. Dilks, K., Kissinger, J. C., T. Brüser, R.W. Rose, and M. Pohlschroder. Heterologous Protein Production. US Patent No. 7,447, 595.

DEVELOPMENT OF COMPUTER PROGRAMS (http://signalfind.org)

  1. Pfeiffer F, Z. Chen, and M. Pohlschroder. 2010. TatLipo, a perl program that predicts prokaryotic Tat lipoproteins.
  2. Imam, S., Z. Zhu and M. Pohlschroder. 2009. PilFind, a perl program that predicts bacterial type IV pilin-like proteins.
  3. Oliveira A, Z. Szabo, S. Albers, A. Driessen, J. Kissinger and M. Pohlschroder. 2006. FlaFind, a perl program that predicts archaeal type IV pilin-like proteins.
  4. Dilks, K., J.C. Kissinger, T. Brüser, R.W. Rose, and M. Pohlschroder. 2002. TatFind, a perl program that predicts prokaryotic Tat signal sequences.

 

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