Publications

Publications

    1. Birol M., D-Muñoz I.I., Rhoades E. (2023) “The C-terminus of α-Synuclein Regulates its Dynamic Cellular Internalization by Neurexin 1β” MBoC, 34: 13 »
    2. Ramirez J., Pancoe S.X., Rhoades E., Petersson E.J. (2023) “The Effects of Lipids on α-Synuclein Aggregation In Vitro” Biomolecules, 13: 1476 »
    3. Galesic A., Pan B., Ramirez J., Rhoades E., Pratt M.R., Petersson E.J. (2023) “Combining non-canonical amino acid mutagenesis and native chemical ligation for multiply modifying proteins: A case study of α-synuclein post-translational modifications” Methods, 218: 101 »
    4. Pan B., Gardner S.M., Schultz K., Perez R.M., Deng S., Shimogawa M., Sato K., Rhoades E., Marmorstein, R., Petersson E.J. (2023) “Semi-Synthetic CoA-α-Synuclein Constructs Trap N-Terminal Acetyltransferase NatB for Binding Mechanism Studies” J. Am. Chem. Soc., 145: 14019 »
    5. Cario A., Wickramasinghe S. P., Rhoades E., Berger C.L. (2022) “N-terminal disease-associated R5L Tau mutation increases microtubule shrinkage rate due to disruption of microtubule-bound Tau patches” Journal of Biological Chemistry, 7: 52 »
    6. Pan B., Shimogawa M., Zhao J., Rhoades E., Kashina, A., Petersson, E.J. (2022) “Cysteine-Based Mimic of Arginylation Reproduces Neuroprotective Effects of the Authentic Post-Translational Modification on α-Synuclein” J. Am. Chem. Soc., 144: 7911-7918 »
    7. Braun A. R., Liao E. E., Horvath M., Kalra R., Acosta K., Young M.C., Kochen N. N., Lo C. H., Brown R., Evans M.D., Pomerantz W. C. K., Rhoades E., Luk K., Cornea R. L., Thomas D. D. and Sachs J. N. (2021) “Potent inhibitors of toxic alpha-synuclein identified via cellular time-resolved FRET biosensors” NPJ Parkinson’s Disease, 7: 52 »
    8. Pan B., Kamo N., Shimogawa M., Huang Y., Kashina A., Rhoades E. and Petersson E. J. (2020) “Effects of Glutamate Arginylation on α-Synuclein: Studying an Unusual Post-Translational Modification through Semisynthesis” J. Am. Chem. Soc., 142(52): 21786-21798 »
    9. Pan B., Park J. H., Ramlall T., Eliezer D., Rhoades E. and Petersson E. J. (2020) “Chemoenzymatic Semi-synthesis Enables Efficient Production of Isotopically-Labeled α-Synuclein with Site-specific Tyrosine Phosphorylation” ChemBioChem »
    10. Castle B. T., McKibben K. M., Rhoades E. and Odde D. J. (2020) “Tau avoids the GTP cap at growing microtubule plus ends” iScience, 23: 101782 »
    11. Wickramasinghe S. P. and Rhoades E. (2020) “Measuring Interactions between Tau and Aggregation Inducers with Single Molecule Förster Resonance Energy Transfer” Methods in Molecular Biology, 2121: 755-775 »
    12. Knox S.L., Steinauer A., Alpha-Cobb G., Trexler A. J., Rhoades E. and Schepartz A. (2020) “Quantification of protein delivery in live cells using fluorescence correlation spectroscopy” Methods in Enzymology, 641: 477-500 »
    13. Pan B., Rhoades E. and Petersson E. J. (2020) “Chemoenzymatic Semisynthesis of Phosphorylated α-Synuclein Enables Identification of a Bidirectional Effect on Fibril Formation” ACS Chemical Biology, 15(3): 640-645 »
    14. Metskas L.A. and Rhoades E. (2020) “Single Molecule FRET of IDPs” Annual Review of Physical Chemistry, 71: 391-414 »
    15. Fung H. Y. J., McKibben K. M., Ramirez J., Gupta K. and Rhoades E. (2020) “Structural Characterization of Tau in Tau:Tubulin Complexes” Structure, 28(3): 378-384 »
    16. McKibben K. and Rhoades E. (2019) “Independent tubulin binding and polymerization by the proline-rich region of Tau is regulated by Tau’s N-terminal domain” Journal of Biological Chemistry, 294: 19381-19394 »
    17. Wickramasinghe S. P., Lempart J., Merens H., Murphy J., Jakob U. and Rhoades E. (2019) “Polyphosphate initiates tau aggregation through intra- and intermolecular cross-linking” Biophysical Journal, 117(4): 717-728 »
    18. Lo C. H., Lim C. K.-W., Ding Z., Wickramasinghe S. P.,  Braun A. R., Rhoades E., Thomas D. D. and Sachs J. N. (2019) “Targeting the ensemble of heterogeneous tau oligomers in cells: A novel small molecule screening platform for tauopathies” in press at Alzheimer’s & Dementia »
    19. Birol M., Wójcik S., Miranker D., Rhoades E., Miranker A. D. (2019) “Identification of N-linked glycans as specific mediators of neuronal uptake of acetylated α-Synuclein” PLoS Biology »
    20. Daniels M., Nourse Jr B., Kim H., Schiavina M., Sainati V., Murrali M. G., Pan B., Ferrie J. J., Haney C., Moons R., Gould N. S., Natalello A., Grandori R., Sobott F., Petersson E. J., Rhoades E., Pierattelli R., Felli I., Uversky V., Caldwell K., Caldwell G., Krol E., and Ischiropoulos H. (2019) “Cyclized NDGA modifies dynamic α-synuclein monomers preventing aggregation and toxicity” Scientific Reports, 9: 2937 »
    21. Wójcik S., Birol M., Rhoades E., Miranker A. D., and Levine Z. A. (2018) “Targeting the Intrinsically Disordered Proteome Using Small-Molecule Ligands” Methods in Enzymology, 611: 703-734 »
    22. Espinoza Sanchez S., Metskas L. A., Chou S. Z., Rhoades E., Pollard T.D. (2018) “Conformational changes in Arp2/3 complex induced by ATP, WASp-VCA and actin filaments” Proceedings of the National Academy of Sciences, 115: E8642-E8651 »
    23. Birol M., Kumar S., Rhoades E., Miranker A.D. (2018) “Conformational switching within dynamic oligomers underpins toxic gain-of-function by diabetes-associated amyloid” Nature Communications, 9: 1312 »
    24. Fung H. Y. J., Birol M., Rhoades E. (2018) “IDPs in macromolecular complexes: the roles of multivalent interactions in diverse assemblies” Current Opinion in Structural Biology, 49: 36-43 »
    25. Ferrie J.J., Haney C.M., Yoon J.,  Pan B., Lin, Y.C., Fakhraai, Z., Rhoades E. , Nath, A., Petersson, E.J. (2018) “Using a FRET Library with Multiple Probe Pairs to Drive Monte Carlo Simulations” Biophysical Journal, 114: 53-64 »
    26. Quach K., LaRochelle J., Li X.-H.,  Rhoades E. , Schepartz, A. (2017) “Unique arginine array improves cytosolic localization of hydrocarbon-stapled peptides” Bioorganic Medicinal Chemistry»
    27. Melo A. M.,  Elbaum-Garfinkle S., Rhoades E. (2017) “Insights into tau function and dysfunction through single-molecule fluorescence” Methods in Cell Biology, 141: 27-44 »
    28. Li X.-H.,  Rhoades E. (2017) “Heterogeneous tau-tubulin complexes accelerate microtubule polymerization” Biophys. J., 112: 2567-2574 »
    29. Braun A.R., Lacy M.M., Ducas V.C., Rhoades E. and Sachs J.N. (2017) “α-Synuclein’s Uniquely Long Amphipathic Helix Enhances its Membrane Binding and Remodeling Capacity.” J. Membr. Biol., 250: 183-193. »
    30. Metskas L. A., Rhoades E. (2016) “Order-Disorder Transitions in the Cardiac Troponin Complex” Journal of Molecular Biology, 428: 2965-2977 »
    31. Melo A. M., Coraor J., Alpha-Cobb G., Elbaum-Garfinkle S., Nath A., Rhoades E. (2016) “Conformational Plasticity in the Tau-Tubulin Complex: A Functional Role for Intrinsic Disorder” Proc. Natl. Acad. Sci. USA, 113: 14336-14341. »
    32. Kumar S., Birol M., Schlamadinger D.E., Rhoades E. and Miranker A.D. (2016) “Foldamer mediated manipulation of a pre-amyloid toxin” Nat. Comm., 7: 11412. »
    33. Tavoulari S., Margheritis E., Nagarajan A., DeWitt D.C., Zhang Y-W., Rosado E., Ravera S., Rhoades E., Forrest L.R., Rudnick G. (2015) “Na+ Sites Control Conformational Change in a Neurotransmitter Transporter Homolog” J. Biol. Chem., 291: 1456-1471. »
    34. Metskas L. A., Rhoades E. (2015) “Conformation and Dynamics of the Troponin I C-Terminal Domain: Combining Single-Molecule and Computational Approaches for a Disordered Protein Region” J. Am. Chem. Soc., 137: 11962-11969. »
    35. Li X.-H., Culver J. A., Rhoades E. (2015) “Tau Binds to Multiple Tubulin Dimers with Helical Structure” J. Am. Chem. Soc., 137: 9218-9221. »
    36. Nath A.,  Schlamadinger D.E., Rhoades E., Miranker A.D.  (2015) “Structure-based small molecule modulation of a pre-amyloid state: pharmacological enhancement of IAPP membrane-binding and toxicity” Biochemistry, 349: 54-58. »
    37. Jülich D., Cobb G., Melo A. M., McMillen P., Lawton A., Mochrie S. G. J., Rhoades E., Holley S. A. (2015) “Cross-scale Integrin regulation organizes ECM and tissue topology” Dev. Cell 34(1): 33-44. »
    38. Metskas L. A., Rhoades E. (2015) “Folding upon phosphorylation: translational regulation by a disorder-to-order transition” Trends Biochem. Sci. 40: 243-244. »
    39. Kumar S., Schlamadinger D. E., Brown M. A., Dunn J. M., Mercado. B., Hebda J. A., Saraogi I., Rhoades E., Hamilton A. D., Miranker A. D. (2015) “Islet Amyloid-Induced Cell Death and Bilayer Integrity Loss Share a Molecular Origin Targetable with Oligopyridylamide-Based α-Helical Mimetics” Chem. Biol. 22: 369-378. »
    40. Liu Y., Cai Y., Liu W., Li X.-H., Rhoades E., Yan E. C. Y. (2015) “Triblock peptide–linker–lipid molecular design improves potency of peptide ligands targeting family B G protein-coupled receptors” Chem. Commun. 51: 6157-6160. »
    41. Esposito D. L. A., Nguyen J. B., DeWitt D. C., Rhoades E., Modis Y. (2015) “Physicochemical requirements and kinetics of membrane fusion of flavivirus-like particles” J. Gen. Virol. vir.0.000113 »
    42. LaRochelle J. R., Cobb G., Steinauer A., Rhoades E., Schepartz A. (2015) “Fluorescence correlation spectroscopy reveals highly efficient cytosolic delivery of certain penta-arg proteins and stapled peptides” J. Am. Chem. Soc., 137: 2536-2541. »
    43. Shi Z., Sachs J. N., Rhoades E., Baumgart T. (2015) “Biophysics of α-synuclein induced membrane remodelling” Phys. Chem. Chem. Phys. Advance Article. »
    44. Ciubotaru M., Surleac M. D., Metskas L. A., Koo P., Rhoades E., Petrescu A. J., Schatz D. G. (2015) “The architecture of the 12RSS in V(D)J recombination signal and synaptic complexes” Nucleic Acids Res. 43: 917-931. »
    45. Braun A. R., Lacy M. M., Ducas V. C., Rhoades E., Sachs J. N. (2014) “α-Synuclein-Induced Membrane Remodeling Is Driven by Binding Affinity, Partition Depth, and Interleaflet Order Asymmetry” J. Am. Chem. Soc., 136: 9962-9972. »
    46. Elbaum-Garfinkle S., Cobb G., Compton J. T., Li X.-H., Rhoades E. (2014) “Tau mutants bind tubulin heterodimers with enhanced affinity” Proc. Natl. Acad. Sci. USA 111: 6311-6316. »
    47. Munro J. B., Nath A., Färber M., Datta S. A. K., Rein A., Rhoades E., Mothes W.(2014) “A conformational transition in HIV-1 Gag early during virus assembly” J. Virol., 88: 3577-3585. »
    48. Fribourgh J., Wolfe L., Nguyen H., DeWitt D. C., Rhoades E., Xiong Y. (2014) “CBFβ plays a critical role facilitating the assembly of the Vif-Cul5 E3 ubiquitin ligase” J. Virol., 88: 3309-3319 »
    49. Ducas V. C., Rhoades E. (2014) “Investigation of intramolecular dynamics and conformations of α-, β-, and γ-Synuclein” PLoS ONE, 9: e86983 »
    50. DeWitt D. C., Rhoades E. (2013) “α-Synuclein Can Inhibit SNARE-Mediated Vesicle Fusion through Direct Interactions with Lipid Bilayers.” Biochemistry 52: 2385-2387.»*Highlighted on the Biochemistry homepage.
    51. Nath A., Rhoades E. (2013) “A Flash in the Pan: Dissecting Dynamic Amyloid Intermediates Using Fluorescence.” FEBS Lett. 587:1096-1105.»
    52. Capraro B. R., Shi Z., Dunn J. M., Wu T., Rhoades E., Baumgart T. (2013) “Kinetics of endophilin N-BAR domain dimerization and membrane interactions” J. Biol. Chem., 288: 12533-12543. »
    53. Ciubotaru M., Trexler A., Spiridon L. N., Surleac M. D., Rhoades E., Petrescu A. J., Schatz D. G. (2013) “RAG and HMGB1 Create a Large Bend in the 23RSS in the V(D)J Recombination Synaptic Complexes.” Nucleic Acids Res. 41:2437-2454. »
    54. Trexler A. J., Rhoades E. (2012) “Function and dysfunction of α-Synuclein: Probing conformational changes and aggregation by single molecule fluorescence” Mol. Neurobiol., 47: 622-631. »
    55. Smith W. W., Schreck C. F., Hashem N., Soltani S., Nath A., Rhoades E., O’Hern C. S. (2012) “Molecular Simulations of the Fluctuating Conformational Dynamics of Intrinsically Disordered Proteins.” Phys. Rev. E. 86:041910. »
    56. Elbaum-Garfinkle S., Rhoades E. (2012) “Identification of an Aggregation-Prone Structure of Tau.” J. Am. Chem. Soc. 134: 16607-16613. »* Highlighted in C&E News, JACS Spotlights, and ASBMB Today.
    57. Nath A., Sammalkorpi M., DeWitt D. C., Trexler A., Elbaum-Garfinkle S., O’Hern C. S., Rhoades E. (2012) “The Conformational Ensembles of α-Synuclein and Tau: Combining Single-Molecule FRET and Simulations.” Biophys. J. 103: 1940-1949. »
    58. Ducas V. C., Rhoades E. (2012) “Quantifying Interactions of β-Synuclein and γ-Synuclein with Model Membranes.” J. Mol. Biol. 423: 528-539. »
    59. Trexler A. J., Rhoades E. (2012) “N-terminal Acetylation is Critical for Forming α-helical Oligomer of α-Synuclein.” Protein Sci. 21: 601-605. »* Most cited paper in Protein Science 2012.
    60. Braun A. R., Sevcsik E., Chin P., Rhoades E., Tristram-Nagle S, Sachs JN. (2012) “α-Synuclein Induces Both Positive Mean Curvature and Negative Gaussian Curvature in Membranes.” J. Am. Chem. Soc. 134: 2613-2620. »
    61. Tseng C.-H., Weinacht T. C., Rhoades E., Murray M., Pearson B. J. (2011) “Using shaped ultrafast laser pulses to detect enzyme binding” Opt. Express, 19: 24638-24646. »
    62. Nath A., Miranker A. D., Rhoades E. (2011) “A Membrane-bound Antiparallel Dimer of Rat Islet Amyloid Polypeptide.” Angew. Chem. Intl. Ed. 50: 10859-10862. »
    63. Last N., Rhoades E., Miranker A. D. (2011) “Islet Amyloid Polypeptide Demonstrates a Persistent Capacity to Disrupt Membrane Integrity.”Proc. Natl. Acad. Sci. USA 108: 9460-9465. »
    64. Sevcsik E., Trexler A. J., Dunn J. M., Rhoades E. (2011) “Allostery in a Disordered Protein: Oxidative Modifications to α-Synuclein Act Distally to Regulate Membrane Binding.” J. Am. Chem. Soc. 133: 7152-7158. »
    65. Cao W., Coman M. M., Ding S., Henn A., Middleton E. R., Bradley M., Rhoades E., Hackney D. D., Pyle A. M., De La Cruz E. M. (2011) “Mechanism of Mss116 ATPase Reveals Functional Diversity of DEAD-Box Proteins.” J. Mol. Biol. 409: 399-414. »
    66. Trexler A. J., Rhoades E. (2010) “Single Molecule Characterization of α-Synuclein in Aggregation-Prone States.” Biophys J. 99: 3048-3055. »*Selected as a “Research Highlight” by Biophysical Journal
    67. Nath A., Trexler A. J., Koo P., Miranker A. D., Atkins W. M., Rhoades E. (2010) “Single-Molecule Fluorescence Spectroscopy Using Phospholipid Bilayer Nanodiscs.” Methods in Enzymology 472: 89-117. »
    68. Middleton E. R., Rhoades E. (2010) “Effects of Curvature and Composition on α-Synuclein Binding to Lipid Vesicles.” Biophys J. 99: 2279-2288. »
    69. Elbaum-Garfinkle S., Ramlal T., Rhoades E. (2010) “The Role of the Lipid Bilayer in Tau Aggregation.” Biophys J. 98: 2722-2730. »
    70. Ilagan R. P., Rhoades E., Gruber D. F., Kao H. T., Pieribone V. A., Regan L. (2010) “A New Bright Green-Emitting Fluorescence Protein – Engineered Monomeric and Dimeric Forms.” FEBS J. 277: 1967-1978 »
    71. Hsiao H. H., Nath A., Lin C.-Y., Folta-Stogniew E. J., Rhoades E., Braddock D. T. (2010) “Quantitative Characterization of the Interactions between c-myc Transcriptional Regulators FUSE, FBP and FIR.” Biochemistry 49: 4620-4634. »
    72. Trexler A. J., Rhoades E. (2009) “α-Synuclein Binds Large Unilamellar Vesicles as an Extended Helix.” Biochemistry 48: 2304-2306 »*One of Biochemistry’s “Most-Accessed Rapid Reports” in 2009
    73. E. Sherman, A. Itkin, Y.Y. Kutner, E. Rhoades, D. Amir, E. Haas, and G. Haran (2008) “Using fluorescence correlation spectroscopy to study conformational changes in denatured proteins” Biophys. J. 94: 4819-4827. »
    74. Nath A., Koo P. K., Rhoades E., Atkins WM. (2008) “Allosteric Effects in Substrate Dissociation from Cytochrome P450 3A4 in Nanodiscs Observed by Ensemble and Single-Molecule Fluorescence Spectroscopy.” J. Am. Chem. Soc. 130:15746-15747 »
    75. Chen H., Rhoades E. (2008) “Fluorescence Characterization of Denatured Proteins.” Curr. Opin. Struct. Biol. 18: 516-524 »